CCL24 antibody (Biotin)

Catalog No. ABIN181597

This Goat Polyclonal antibody specifically detects CCL24 in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for CCL24 antibody (Biotin) (ABIN181597)

Target: CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CCL24 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CCL24 Antibody (Biotin)

Specificity: This antibody detects Eotaxin-2.

Purification: Affinity chromatography

Immunogen: Highly pure (> 98 %) recombinant human Eotaxin-2

Application Details

Application Notes: ELISA: Direct: To detect Eotaxin-2 by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. In conjunction with compatible secondaryreagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Eotaxin-2. Western Blot: Sandwich: To detect Eotaxin-2 by sandwich ELISA (using 100 μL/wellantibody solution) a concentration of 0.25 - 1.0 μg/mL is required. In conjunction withPolyclonal Anti-Human Eotaxin-2 as a capture antibody, it allows the detection of at least0.2 - 0.4 ng/well of recombinant Eotaxin-2.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for CCL24

Target: CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))

Alternative Name: Eotaxin-2 / CCL24

Background: Eotaxin 2 is a member of the CC chemokine family, based on the presence of the CC motif and homology with other known CC chemokines. Eotaxin 2 cDNA encodes a 119 amino acid residue precursor protein with a 26 amino acid residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N glycosylation site. Mature human eotaxin 2 has a predicted molecular mass of approximately 10.6 kDa. Compared to other CC chemokines, eotaxin 2 exhibits 40 %, 42 %, and 39 % amino acid identity to MCP3, MIP1 alpha, and eotaxin, respectively. Human CC chemokine eotaxin 2 maps to chromosome 7q11.23. Both eotaxin and eotaxin 2 activate and attract eosinophils and basophils. A receptor for human eotaxin has been identified and found to be the third numbered receptor in the C-C chemokine subfamily of receptors (CCR3. On eosinophils, the effects of eotaxin 2 is inhibited by an CCR3 antibody and cross-desensitized by eotaxin and MCP4, suggesting that all three CC chemokines act through CCR3. Eotaxin 2 mRNA is weakly expressed in activated monocytes and T lymphocytes. Recombinant eotaxin 2 induces chemotaxis of eosinophils, basophils, and resting T lymphocytes but not monocytes and activated T lymphocytes. Eotaxin 2 inhibits colony formation in myleloid progenitor cells.Synonyms: C-C motif chemokine 24, CK-beta-6, Eosinophil chemotactic protein 2, MPIF-2, MPIF2, Myeloid progenitor inhibitory factor 2, SCYA24, Small-inducible cytokine A24

Gene ID: 6369

NCBI Accession: NP_002982

UniProt: O00175

Pathways: Regulation of Actin Filament Polymerization