CCL22 antibody (Biotin)

Catalog No. ABIN181601

The Rabbit Polyclonal anti-CCL22 antibody has been validated for WB and EIA. It is suitable to detect CCL22 in samples from Mouse.

Quick Overview for CCL22 antibody (Biotin) (ABIN181601)

Target: CCL22 (Chemokine (C-C Motif) Ligand 22 (CCL22))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL22 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CCL22 Antibody (Biotin)

Specificity: This antibody detects MDC.

Purification: Affinity Chromatography

Immunogen: Highly pure (>98%) E.coli derived recombinant Murine MDC

Application Details

Application Notes: ELISA: Direct: To detect mMDC by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMDC. Sandwich: To detect mMDC by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. In conjunction with Polyclonal Anti-MurineMDC as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant mMDC. Western Blot: To detect mMDC by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mMDC is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for CCL22

Target: CCL22 (Chemokine (C-C Motif) Ligand 22 (CCL22))

Alternative Name: MDC / CCL22

Background: ABCD1 belongs to the ALD subfamily, of the superfamily of ATP-binding cassette (ABC) transporters and is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. All known peroxisomal ABC transporters are half transporters which require a partner half transporter molecule to form a functional homodimeric or heterodimeric transporter. This peroxisomal membrane protein is likely involved in the peroxisomal transport or catabolism of very long chain fatty acids. Defects in this gene have been identified as the underlying cause of adrenoleukodystrophy, an X-chromosome recessively inherited demyelinating disorder of the nervous system.Synonyms: C-C motif chemokine 22, CC chemokine STCP-1, Macrophage-derived chemokine, SCYA22, Small-inducible cytokine A22, Stimulated T-cell chemotactic protein 1

Gene ID: 20299

NCBI Accession: NP_033163

UniProt: O88430