IL-19 antibody

Catalog No. ABIN181616

This Rabbit Polyclonal antibody specifically detects IL-19 in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for IL-19 antibody (ABIN181616)

Target: IL-19 (IL19) (Interleukin 19 (IL19))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-19 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-IL-19 Antibody

Specificity: This antibody detects Interleukin-19.

Purification: Affinity Chromatography employing immobilized Human IL19 matrix.

Immunogen: Highly pure (>98%) E.coli derived recombinant Human IL-19

Application Details

Application Notes: ELISA: Indirect: To detect hIL-19 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. In conjunction withcompatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hIL-19. Sandwich: To detect hIL-19 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. In conjunction with Biotinylated Anti-HumanIL-19 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hIL-19. Western Blot: To detect hIL-19 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-19 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution, the product ist stable at 2-8 °C for up one week or (in aliquots) at -20 °C for up to six months.

Target Details for IL-19

Target: IL-19 (IL19) (Interleukin 19 (IL19))

Alternative Name: Interleukin-19 / IL19

Background: IL19 is a cytokine that belongs to the IL10 cytokine subfamily. This cytokine is found to be preferentially expressed in monocytes. It can bind the IL20 receptor complex and lead to the activation of the signal transducer and activator of transcription 3 (STAT3). A similar cytokine in mouse is reported to up-regulate the expression of IL6 and TNF-alpha and induce apoptosis, which suggests a role of this cytokine in inflammatory responses. Little is known about the biologic function and gene regulation of IL19. Alternatively spliced transcript variants encoding distinct isoforms have been described.Synonyms: IL-19, Melanoma differentiation-associated protein-like protein, NG.1, ZMDA

Gene ID: 29949

NCBI Accession: NP_037503

UniProt: Q9UHD0