Nanog antibody (Biotin)

Catalog No. ABIN181657

This Rabbit Polyclonal antibody specifically detects Nanog in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for Nanog antibody (Biotin) (ABIN181657)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nanog antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Nanog Antibody (Biotin)

Specificity: This antibody recognizes Human Nanog. Other species not tested.

Purification: Affinity Chromatography

Immunogen: Highly pure (> 98%) E.coli recombinant Human Nanog

Application Details

Application Notes: ELISA: Direct: To detect hNanog (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNanog. Sandwich: To detect hNanog (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-HumanNanog as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hNanog. Western blot: To detect hNanog this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hNanog is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Alternative Name: NANOG

Background: Nanog is a newly identified homeodomain-bearing transcriptional factor. Nanog expression is specific to early embryos and pluripotential stem cells including mouse and human embryonic stem (ES) and embryonic germ (EG) cells. It is a key molecule involved in the signaling pathway for maintaining the capacity for self-renewal and pluripotency, bypassing regulation by the STAT3 pathway. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Nanog-deficient ES cells lose pluripotency and differentiate into extraembryonic endoderm lineage. Thus it is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human. NANOG is a new marker for testicular carcinoma in situ and germ cell tumors.Synonyms: Homeobox protein NANOG

Gene ID: 100293888

NCBI Accession: NP_079141

UniProt: Q9H9S0

Pathways: Stem Cell Maintenance