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APOE antibody (Isoform E3) (Biotin)

This anti-APOE antibody is a Rabbit Polyclonal antibody detecting APOE in WB and EIA. Suitable for Human.
Catalog No. ABIN181665

Quick Overview for APOE antibody (Isoform E3) (Biotin) (ABIN181665)

Target

See all APOE Antibodies
APOE (Apolipoprotein E (APOE))

Reactivity

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Human

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This APOE antibody is conjugated to Biotin

Application

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Western Blotting (WB), Enzyme Immunoassay (EIA)
  • Binding Specificity

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    Isoform E3

    Specificity

    This antibody detects ApoE3. Cross reacts with Human ApoE2 and Human ApoE4 in both Sandwich ELISA and Western Blot.

    Purification

    Affinity Chromatography.

    Immunogen

    E.Coli derived recombinant Human ApoE3.
  • Application Notes

    Direct ELISA: To detect Human ApoE3 by Direct ELISA (using 100 μL/well antibody solution)a concentration of approx. 1.0 μg/mL of this antibody is required. This Biotinylated polyclonalantibody allows the detection of at least 0.2-0.4 ng/well of recombinant Human ApoE3. Sandwich ELISA: To detect Human ApoE3 by Sandwich ELISA (using 100 μL/well) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This Biotinylated antibody, inconjunction with Affinity purified anti-Human ApoE3 (ABIN181667 or ABIN181668) as acapture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant HumanApoE3. Western blot: To detect Human ApoE3 this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant Human ApoE3 is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Reconstitution

    Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

    Buffer

    PBS, pH 7.2 without preservatives.

    Preservative

    Without preservative

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    -20 °C

    Storage Comment

    Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

    Expiry Date

    6 months
  • Target

    APOE (Apolipoprotein E (APOE))

    Alternative Name

    Apolipoprotein E (Apo E)

    Background

    Apolipoprotein E3 mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo E receptor (chylomicron remnant) of hepatic tissues.Synonyms: Apo-E, ApoE

    Gene ID

    348

    NCBI Accession

    NP_000032

    UniProt

    P02649

    Pathways

    Regulation of Cell Size, Lipid Metabolism
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