IL28A antibody (Biotin)

Catalog No. ABIN181685

The Rabbit Polyclonal anti-IL28A antibody has been validated for WB and EIA. It is suitable to detect IL28A in samples from Human.

Quick Overview for IL28A antibody (Biotin) (ABIN181685)

Target: IL28A (Interleukin 28A (Interferon, lambda 2) (IL28A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL28A antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-IL28A Antibody (Biotin)

Specificity: This antibody detects Interferon-lambda 2.

Purification: Affinity chromatography

Immunogen: Highly pure (> 98 %) recombinant human IFN-lambda2

Application Details

Application Notes: ELISA: Direct: To detect hIFN-λ2 (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIFN-λ2. Sandwich: To detect hIFN-λ2(using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-HumanIFN-λ2 as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hIFN-λ2. Western blot: To detect hIFN-λ2 this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hIFN-λ2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for IL28A

Target: IL28A (Interleukin 28A (Interferon, lambda 2) (IL28A))

Alternative Name: Interleukin-28A / IL28A

Background: IL28 is a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28B (IL28B), and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA).Synonyms: IFNL2, IL-28A, Interferon lambda-2, ZCYTO20

Gene ID: 282616

NCBI Accession: NP_742150

UniProt: Q8IZJ0