IL-31 antibody (Biotin)

Catalog No. ABIN181693

This Rabbit Polyclonal antibody specifically detects IL-31 in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for IL-31 antibody (Biotin) (ABIN181693)

Target: IL-31 (IL31) (Interleukin 31 (IL31))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-31 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-IL-31 Antibody (Biotin)

Specificity: This antibody detects Interleukin-31.

Purification: Affinity chromatography

Immunogen: Highly pure (> 98 %) recombinant human IL-31

Application Details

Application Notes: ELISA: Direct: To detect hIL-31 (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-31. Sandwich: To detect hIL-31 (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-HumanIL-31 as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hIL-31. Western blot: To detect hIL-31 this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hIL-31 is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for IL-31

Target: IL-31 (IL31) (Interleukin 31 (IL31))

Alternative Name: Interleukin-31 / IL31

Background: Interleukin-31 (IL-31) is a recently discovered T-cell cytokine closely related to IL-6 type cytokines and is preferentially produced by T helper type 2 cells. IL-31 activity is mediated through the ligand-induced oligomerization of a dimeric receptor complex containing IL-31 receptor A and oncostatin M receptor. In response to IL-31 binding, these proteins activate the JAK/STAT and the AKT signaling pathways. RNA levels of IL-31 receptor A and oncostatin M receptor are induced in activated monocytes but are expressed constitutively in epithelial cells. IL-31, when overexpressed in transgenic mice, results in the development of pruritis, alopecia and skin lesions, and in humans may result in atopic dermatitis, suggesting that IL-31 may represent a novel target for antipruritic drug development.Synonyms: IL-31

Gene ID: 386653

NCBI Accession: NP_001014358

UniProt: Q6EBC2