CTGF antibody (Biotin)

Catalog No. ABIN181697

The Rabbit Polyclonal anti-CTGF antibody has been validated for WB and EIA. It is suitable to detect CTGF in samples from Human.

Quick Overview for CTGF antibody (Biotin) (ABIN181697)

Target: CTGF (Connective Tissue Growth Factor (CTGF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CTGF antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CTGF Antibody (Biotin)

Specificity: This antibody detects Connective Tissue Growth Factor.

Purification: Affinity Chromatography

Immunogen: Highly pure (> 98%) recombinant Human CTGF

Application Details

Application Notes: ELISA: Direct: To detect hCTGF (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCTGF. Sandwich: To detect hCTGF (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-HumanCTGF as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hCTGF. Western blot: To detect hCTGF this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hCTGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years from date of receipt at -20 °C. Store the reconstituted antibody at 2-8 °C for one month or at -20 °C (in aliquots) for longer.

Expiry Date: 12 months

Target Details for CTGF

Target: CTGF (Connective Tissue Growth Factor (CTGF))

Alternative Name: CTGF

Background: CTGF (connective tissue growth factor) is a 38 kDa, cysteine-rich, secreted peptide. It is a new member of the peptide family that include serum-induced immediate early gene products, a v-src-induced peptide and a putative proto-oncogene. Among the many functions of the CTGF gene family are embryogenesis, wound healing and regulation of extracellular matrix production.Synonyms: CCN family member 2, CCN2, Connective tissue growth factor, HCS24, Hypertrophic chondrocyte-specific protein 24, IBP8, IGF-binding protein 8, IGFBP8, Insulin-like growth factor-binding protein 8

Gene ID: 1490

NCBI Accession: NP_001892

UniProt: P29279

Pathways: Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Growth Factor Binding