CTGF antibody

Catalog No. ABIN181700

This Rabbit Polyclonal antibody specifically detects CTGF in WB, IHC (p), EIA and Func. It exhibits reactivity toward Human.

Quick Overview for CTGF antibody (ABIN181700)

Target: CTGF (Connective Tissue Growth Factor (CTGF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CTGF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-CTGF Antibody

Specificity: This antibody detects Human Connective Tissue Growth Factor (CTGF). Other species not tested.

Purification: Immunoaffinity Chromatography employing immobilized Human CTGF matrix.

Immunogen: Highly pure (>98%) E.coli derived recombinant Human CTGF.

Application Details

Application Notes: Neutralisation: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman CTGF (5.0 μg/mL) a concentration of 6.0-9.0 μg/mL of this antibody is required. Sandwich ELISA: To detect Human CTGF (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This affinity purified antibody,in conjunction with Biotinylated antibody Human CTGF (AP01150BT) as a detectionantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human CTGF. Western blot: To detect Human CTGF this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant hCTGF is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL. Centrifuge vial prior to opening.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CTGF

Target: CTGF (Connective Tissue Growth Factor (CTGF))

Alternative Name: CTGF

Background: CTGF (connective tissue growth factor) is a 38 kDa, cysteine-rich, secreted peptide. It is a new member of the peptide family that include serum-induced immediate early gene products, a v-src-induced peptide and a putative proto-oncogene. Among the many functions of the CTGF gene family are embryogenesis, wound healing and regulation of extracellular matrix production.Synonyms: CCN family member 2, CCN2, Connective tissue growth factor, HCS24, Hypertrophic chondrocyte-specific protein 24, IBP8, IGF-binding protein 8, IGFBP8, Insulin-like growth factor-binding protein 8

Gene ID: 1490

NCBI Accession: NP_001892

UniProt: P29279

Pathways: Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Growth Factor Binding