CD5 antibody (PE)

Catalog No. ABIN181849

This Mouse Monoclonal antibody specifically detects CD5 in FACS. It exhibits reactivity toward Cat.

Quick Overview for CD5 antibody (PE) (ABIN181849)

Target: CD5

Reactivity: Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD5 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: FE1-1B11

Product Details anti-CD5 Antibody (PE)

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Neat - 1/10, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL distilled water

Buffer: PBS containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD5

Target: CD5

Alternative Name: CD5

Background: CD5 is a 55 kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors.Synonyms: LEU-1, LEU1, Ly-1, Lymphocyte antigen T1/Leu-1, Lyt-1, T-cell surface glycoprotein CD5