CD31 antibody

Catalog No. ABIN1834919

The Mouse Monoclonal anti-CD31 antibody has been validated for FACS, IHC, IF and IHC (fro). It is suitable to detect CD31 in samples from Human and Pig.

Quick Overview for CD31 antibody (ABIN1834919)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivity: Human, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD31 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: LCI-4

Product Details anti-CD31 Antibody

Specificity: Recognizes porcine CD31, also known as Platelet endothelial cell adhesion molecule (PECAM-1). CD31 is constitutively expressed by platelets, monocytes and some lymphocytes, it is expressed by endothelial cells at a level, an order of magnitude greater that of other cell types. The extracellular region contains six Ig-like domains. Clone LCI-4 is cross reactive with human CD31 and binds to the 5th extracellular Ig domain, proximal to the transmembrane region as demonstrated by human CD31 domain deletion mutant protein binding studies. Immunoprecipitates a protein of ~130 kD from lysates of porcine aortic endothelial cells and is strongly expressed at cell junctions.

Purification: Protein G purified

Immunogen: Porcine CD31/human IgGFc fusion protein.

Type of Immunogen: Fusion protein

Isotype: IgG1

Application Details

Application Notes: Approved: Flo, IF, IHC, IHC-Fr

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 106 cells or 100 μL whole blood.

Comment:

Target Species of Antibody: Pig

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: PECAM-1 / CD31

Background: Name/Gene ID: PECAM1

Synonyms: PECAM1, CD31, CD31 antigen, EndoCAM, PECA1, PECAM-1, GPIIA

Gene ID: 5175

UniProt: P16284

Pathways: Regulation of Actin Filament Polymerization