CBP Tag antibody

Catalog No. ABIN1842586

The Mouse Monoclonal anti-CBP Tag antibody has been validated for WB and ELISA. It is suitable to detect CBP Tag in samples from . There is 1 publication available.

Quick Overview for CBP Tag antibody (ABIN1842586)

Target: CBP Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This CBP Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 1D11E5

Product Details anti-CBP Tag Antibody

Sequence: KRRWKKNFIA VSAA

Specificity: CBP Tag Antibody, mAb, Mouse recognizes CBP-tagged proteins.

Characteristics: CBP Tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes harvested from mouse immunized with CBP tag peptide conjugated to KLH.

Purification: Protein A affinity column

Immunogen: CBP tag peptide conjugated to KLH

Isotype: IgG2b kappa

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Western blot: 1 µg/mL.
Immunoprecipitation: 1-5 µg/mL.
ELISA: 0. 001-0. 002 µg/mL.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.

Concentration: 0.5 mg/mL

Buffer: lyophilized with PBS, pH 7.4, containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below.

References for anti-CBP Tag antibody (ABIN1842586)

Li, Liu, Zhang, Issaian, Hill, Espinosa, Shi, Cui, Kappel, Das, Hansen, Zhou, Zhao: "A unified mechanism for intron and exon definition and back-splicing." in: Nature, Vol. 573, Issue 7774, pp. 375-380, (2020) (PubMed).

Target Details for CBP Tag

Target: CBP Tag

Target Type: Tag

Background: CBP affinity tag is a useful tag in protein study which can be added to the N or C terminus of proteins of interest through DNA recombinant technology. The tag is derived from muscle myosin light-chain kinase. The tag comprises 26 amino acid residues with the molecular weight of 4 kDa and sequence of KRRWKKNFIAVSAANRFKKISSSGAL. CBP tag has the relatively high affinity for calmodulin (CaM). CBP-tagged proteins can be purified from crude cell extracts through CaM affinity resin. CBP Tag antibody is a useful tool in analysis of CBP-tagged proteins