Dynactin 1 antibody (C-Term)

Catalog No. ABIN184834

This Goat Polyclonal antibody specifically detects Dynactin 1 in WB, ELISA, IHC, IF and FACS. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for Dynactin 1 antibody (C-Term) (ABIN184834)

Target: Dynactin 1 (DCTN1)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Dynactin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-Dynactin 1 Antibody

Binding Specificity: C-Term

Purpose: Dynactin

Sequence: QEQLHQLHSR LIS

Specificity: This antibody is expected to recognise all reported isoforms.

Cross-Reactivity: Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QEQLHQLHSRLIS, from the C Terminus of the protein sequence according to NP_004073.2, NP_075408.1, NP_001128512.1, NP_001128513.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 6-7 μg/mL.

Western Blot: Approx. 150 kDa band observed in HeLa and MCF7 cell lysates, and in preliminary testing of HEK293 and Mouse Brain lysate (calculated MW of 142 kDa according to NP_004073.2). Recommended concentration 0.3-1 μg/mL. Primary incubation 1 hour at

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells and additionally the nucleus of U2OS cells.. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-Dynactin 1 antibody (ABIN184834)

Bupha-Intr, Haizlip, Janssen: "Temporal changes in expression of connexin 43 after load-induced hypertrophy in vitro." in: American journal of physiology. Heart and circulatory physiology, Vol. 296, Issue 3, pp. H806-14, (2009) (PubMed).

Target Details for Dynactin 1

Target: Dynactin 1 (DCTN1)

Alternative Name: DCTN1

Background: DCTN1, dynactin 1 (p150, glued homolog, Drosophila), P135, DP-150, DAP-150, p150-glued, p150, Glued (Drosophila) homolog, 150 kDa dynein-associated polypeptide, dynactin 1 (p150, Glued (Drosophila) homolog), HMN7B, OTTHUMP00000202491, dynactin 1, p150, Gl

Gene ID: 1639, 13191

NCBI Accession: NP_004073, NP_075408, NP_001128512, NP_001128513, NP_001177765, NP_001177766

Pathways: M Phase, ER-Nucleus Signaling