FOXC2 antibody (C-Term)

Catalog No. ABIN185029

This Goat Polyclonal antibody specifically detects FOXC2 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for FOXC2 antibody (C-Term) (ABIN185029)

Target: FOXC2 (Forkhead Box C2 (MFH-1, Mesenchyme Forkhead 1) (FOXC2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FOXC2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-FOXC2 Antibody

Binding Specificity: C-Term

Purpose: FOXC2

Sequence: RHAAPYSYDC TKY

Cross-Reactivity: Cow, Dog, Human, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence RHAAPYSYDCTKY, from the C Terminus of the protein sequence according to NP_005242.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 60-70 kDa band observed in nuclear lysates of cell lines HEK293 (calculated MW of 53.7 kDa according to NP_005242.1). This molecular weight is routinely observed by other sources e.g. Berry, FB et al, PMID: 16081467. Recommended concen

Peptide ELISA: antibody detection limit dilution 1:4000.

Comment:

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for FOXC2

Target: FOXC2 (Forkhead Box C2 (MFH-1, Mesenchyme Forkhead 1) (FOXC2))

Alternative Name: FOXC2

Background: FOXC2, forkhead box C2 (MFH-1, mesenchyme forkhead 1), MFH1, MFH-1, FKHL14, MFH-1,mesenchyme forkhead 1, forkhead (Drosophila)-like 14, forkhead, Drosophila, homolog-like 14, forkhead (Drosophila)-like 14 (MFH-1, mesenchyme forkhead 1), LD, forkhead box C

Gene ID: 2303, 14234

NCBI Accession: NP_005242