MYRIP antibody (C-Term)

Catalog No. ABIN185041

This Goat Polyclonal antibody specifically detects MYRIP in ELISA, WB and IHC. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for MYRIP antibody (C-Term) (ABIN185041)

Target: MYRIP (Myosin VIIA and Rab Interacting Protein (MYRIP))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MYRIP antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-MYRIP Antibody

Binding Specificity: C-Term

Purpose: MYRIP / SLAC2C

Sequence: KDLMEPALES AVMY

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KDLMEPALESAVMY, from the C Terminus of the protein sequence according to NP_056275.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Breast, Colon and Brain (Cortex). Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 90 kDa band observed in Human Brain (Amygdala) lysates (calculated MW of 95.7 kDa according to NP_056275.2). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-MYRIP antibody (ABIN185041)

Huet, Fanget, Jouannot, Meireles, Zeiske, Larochette, Darchen, Desnos: "Myrip couples the capture of secretory granules by the actin-rich cell cortex and their attachment to the plasma membrane." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 7, pp. 2564-77, (2012) (PubMed).

Target Details for MYRIP

Target: MYRIP (Myosin VIIA and Rab Interacting Protein (MYRIP))

Alternative Name: MYRIP

Background: MYRIP, SLAC2C, myosin VIIA and Rab interacting protein, MyRIP, SLAC2-C, exophilin, exophilin 8, DKFZp586F1018, rab effector MYRIP, Slp homologue lacking C2 domains, synaptotagmin-like protein homologue lacking C2 domains-c, FLJ44025, MGC130034, MGC130035,

Gene ID: 25924

NCBI Accession: NP_056275

Pathways: Positive Regulation of Peptide Hormone Secretion