Pleckstrin antibody (C-Term)

Catalog No. ABIN185127

This anti-Pleckstrin antibody is a Goat Polyclonal antibody detecting Pleckstrin in WB, IHC, ELISA, FACS and IF. Suitable for Human.

Quick Overview for Pleckstrin antibody (C-Term) (ABIN185127)

Target: Pleckstrin (PLEK)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Pleckstrin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-Pleckstrin Antibody

Binding Specificity: C-Term

Purpose: Pleckstrin

Sequence: EWIKAIQMAS RTGK

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-EWIKAIQMASRTGK

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Vessel (platelets) and Spleen. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 40-45 kDa band observed in Human Parathyroid gland lysates and also in preliminary testing of Mouse Spleen lysates and lysates of cell line U937 (calculated MW of 40.1 kDa according to Human NP_002655.2). Recommended concentration: 0.0

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes and nuclei of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended co

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Pleckstrin

Target: Pleckstrin (PLEK)

Alternative Name: PLEK

Background: PLEK, pleckstrin, P47, p47, FLJ27168

Gene ID: 5341

NCBI Accession: NP_002655

Pathways: Inositol Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process