MPP5 antibody (N-Term)

Catalog No. ABIN185150

The Goat Polyclonal anti-MPP5 antibody has been validated for ELISA, IHC, FACS and IF. It is suitable to detect MPP5 in samples from Human.

Quick Overview for MPP5 antibody (N-Term) (ABIN185150)

Target: MPP5 (Membrane Protein, Palmitoylated 5 (MAGUK P55 Subfamily Member 5) (MPP5))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MPP5 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-MPP5 Antibody

Binding Specificity: N-Term

Purpose: PALS / MPP5

Sequence: NKASPPFPLI SNAQ

Cross-Reactivity: Cow, Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: NKASPPFPLISNAQ-C

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Small Intestine and Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Preliminary testing showed a band at approx 75 kDa in Human Cerebellum lysate after 1 μg/mL antibody staining (calculated MW of 73.4 kDa according to NP_001243479.1). Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in U2OS and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MPP5

Target: MPP5 (Membrane Protein, Palmitoylated 5 (MAGUK P55 Subfamily Member 5) (MPP5))

Alternative Name: MPP5

Background: MPP5, PALS1, FLJ12615, membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5), stardust (Drosophila), MAGUK p55 subfamily member 5, membrane protein, palmitoylated 5, stardust

Gene ID: 64398

NCBI Accession: NP_071919

Pathways: Nucleotide Phosphorylation, Tube Formation, Asymmetric Protein Localization