TCP1 alpha/CCTA antibody (C-Term)

Catalog No. ABIN185185

This Goat Polyclonal antibody specifically detects TCP1 alpha/CCTA in WB, IHC, ELISA, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for TCP1 alpha/CCTA antibody (C-Term) (ABIN185185)

Target: TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TCP1 alpha/CCTA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-TCP1 alpha/CCTA Antibody

Binding Specificity: C-Term

Purpose: TCP1

Sequence: SYEDAVHSGA LND

Specificity: This antibody is expected to recognise both reported isoforms.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SYEDAVHSGALND, from the C Terminus of the protein sequence according to NP_110379.2, NP_001008897.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Testis shows only cytoplasmic staining in some germ cells, and both cytoplasmic and nuclear staining in other germ cells with unstained nucleoli. Recommended concentration: 1-2 μg/mL.

Western Blot: Approx 60 kDa band observed in lysates of cell line HEK293 and in Human Ovary lysates (calculated MW of 60.3 kDa according to NP_110379.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TCP1 alpha/CCTA

Target: TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

Alternative Name: TCP1

Background: TCP1, t-complex 1, CCT-alpha, CCT1, CCTa, D6S230E, TCP-1-alpha, T-complex protein 1, T-complex protein 1, alpha subunit, tailless complex polypeptide 1

Gene ID: 6950

NCBI Accession: NP_110379, NP_001008897