FHL2 antibody (C-Term)

Catalog No. ABIN185198

The Goat Polyclonal anti-FHL2 antibody has been validated for WB and ELISA. It is suitable to detect FHL2 in samples from Human and Mouse.

Quick Overview for FHL2 antibody (C-Term) (ABIN185198)

Target: FHL2 (Four and A Half LIM Domains 2 (FHL2))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This FHL2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-FHL2 Antibody

Binding Specificity: C-Term

Purpose: FHL2

Sequence: RDDILCPDCG KDI

Specificity: Reported variants represent identical protein (NP_001441.4, NP_963849.1, NP_963851.2, NP_001034581.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence RDDILCPDCGKDI, from the C Terminus of the protein sequence according to NP_001441.4.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 30 kDa band observed in Human and Mouse Heart lysates (calculated MW of 32.2 kDa according to NP_001441.4). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for FHL2

Target: FHL2 (Four and A Half LIM Domains 2 (FHL2))

Alternative Name: FHL2

Background: FHL2, DRAL, AAG11, SLIM3, four and a half LIM domains 2, down-regulated in rhabdomyosarcoma LIM protein, aging-associated gene 11, four and a half LIM-domain protein 2, OTTHUMP00000203164, OTTHUMP00000203168, OTTHUMP00000203169

Gene ID: 2274, 14200, 63839

NCBI Accession: NP_001441

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha