FOXP3 antibody (C-Term)

Catalog No. ABIN185239

This Goat Polyclonal antibody specifically detects FOXP3 in FACS, ELISA, IHC and IF. It exhibits reactivity toward Mouse.

Quick Overview for FOXP3 antibody (C-Term) (ABIN185239)

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This FOXP3 antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Verified

Product Details anti-FOXP3 Antibody

Binding Specificity: C-Term

Purpose: FOXP3 / Scurfin (Mouse)

Sequence: KRSQRPNKCS NP

Specificity: Note - anti-mouse companion product of EB05294

Cross-Reactivity: Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence KRSQRPNKCSNP, from the C Terminus of the protein sequence according to NP_473380.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Mouse Spleen. Recommended concentration: 5-6 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of NIH3T3 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for FOXP3

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Alternative Name: Foxp3

Background: Foxp3, forkhead box P3, sf, JM2, scurfin, scurfy, RP23-54C14.1, OTTMUSP00000017952, OTTMUSP00000017961, OTTMUSP00000017962

Gene ID: 20371, 317382

NCBI Accession: NP_473380

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation