ABCC4 antibody (C-Term)

Catalog No. ABIN185302

This Goat Polyclonal antibody specifically detects ABCC4 in ELISA, IHC. It exhibits reactivity toward Human and has been mentioned in 4+ publications.

Quick Overview for ABCC4 antibody (C-Term) (ABIN185302)

Target: ABCC4 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 4 (ABCC4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ABCC4 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-ABCC4 Antibody

Binding Specificity: C-Term

Purpose: ABCC4 / MRP4

Sequence: CNGQPSTLTI FETAL

Specificity: This antibody is expected to recognize isoform 1 (NP_005836.2) only.

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CNGQPSTLTIFETAL, from the C Terminus of the protein sequence according to NP_005836.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Prostate shows membranous staining in the secretory cells. Recommended concentration, 4-6 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-ABCC4 antibody (ABIN185302)

Gori, Rodriguez, Pellegrini, Achtari, Hornung, Chardonnens, Wunder, Fiche, Canny: "Augmented epithelial multidrug resistance-associated protein 4 expression in peritoneal endometriosis: regulation by lipoxin A(4)." in: Fertility and sterility, Vol. 99, Issue 7, pp. 1965-73.e2, (2013) (PubMed).

Montani, Hermanns, Herrmanns, Müntener, Wild, Sulser, Kristiansen: "Multidrug resistance protein 4 (MRP4) expression in prostate cancer is associated with androgen signaling and decreases with tumor progression." in: Virchows Archiv : an international journal of pathology, Vol. 462, Issue 4, pp. 437-43, (2013) (PubMed).

Weerachayaphorn, Mennone, Soroka, Harry, Hagey, Kensler, Boyer: "Nuclear factor-E2-related factor 2 is a major determinant of bile acid homeostasis in the liver and intestine." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 302, Issue 9, pp. G925-36, (2012) (PubMed).

Xu, Weerachayaphorn, Cai, Soroka, Boyer: "Aryl hydrocarbon receptor and NF-E2-related factor 2 are key regulators of human MRP4 expression." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 299, Issue 1, pp. G126-35, (2010) (PubMed).

Target Details for ABCC4

Target: ABCC4 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 4 (ABCC4))

Alternative Name: ABCC4

Background: ABCC4, MRP4, MOATB, MOAT-B, EST170205, ATP-binding cassette, sub-family C (CFTR/MRP), member 4, canalicular multispecific organic anion transporter (ABC superfamily), Multidrug Resistance Protein 4, RP11-74A12.1, ATP-binding cassette, sub-family C, member

Molecular Weight: 149kDa

Gene ID: 10257, 170924

NCBI Accession: NP_005836