EFHD2 antibody (C-Term)

Catalog No. ABIN185311

This Goat Polyclonal antibody specifically detects EFHD2 in ELISA and WB. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for EFHD2 antibody (C-Term) (ABIN185311)

Target: EFHD2 (EF-Hand Domain Family, Member D2 (EFHD2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This EFHD2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Grade: Verified

Product Details anti-EFHD2 Antibody

Binding Specificity: C-Term

Purpose: Swiprosin 1 / EFHD2

Sequence: CAAFKELQST FK

Specificity: This antibody does not cross-react with Mouse Swiprosin 2. Data kindly provided by Sebastian Duetting and Dirk Mielenz.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: CAAFKELQSTFK

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 30 kDa band observed in Human Thymus, Human Lymph node and Human Tonsil lysates (calculated MW of 26.7 kDa according to NP_077305.2). Recommended concentration: 0.5-1.5 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-EFHD2 antibody (ABIN185311)

Brachs, Lang, Buslei, Purohit, Fürnrohr, Kalbacher, Jäck, Mielenz: "Monoclonal antibodies to discriminate the EF hand containing calcium binding adaptor proteins EFhd1 and EFhd2." in: Monoclonal antibodies in immunodiagnosis and immunotherapy, Vol. 32, Issue 4, pp. 237-45, (2013) (PubMed).

Target Details for EFHD2

Target: EFHD2 (EF-Hand Domain Family, Member D2 (EFHD2))

Alternative Name: EFHD2

Background: EFHD2, MGC4342, EF hand domain containing 2, EF-hand domain family, member D2, RP3-467K16.3, SWS1, EF hand domain family, member D2, swiprosin 1

Gene ID: 79180, 27984

NCBI Accession: NP_077305