KLRK1 antibody (Internal Region)

Catalog No. ABIN185435

The Goat Polyclonal anti-KLRK1 antibody has been validated for WB, FACS and ELISA. It is suitable to detect KLRK1 in samples from Human.

Quick Overview for KLRK1 antibody (Internal Region) (ABIN185435)

Target: KLRK1 (Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This KLRK1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Grade: Verified

Product Details anti-KLRK1 Antibody

Binding Specificity: Internal Region

Purpose: NKG2D / KLRK1

Sequence: KVYSKEDQDL LK

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KVYSKEDQDLLK, from the internal region of the protein sequence according to NP_031386.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 36-37 kDa band observed in lysates of cell line MOLT4 (calculated MW of 25.3 kDa according to NP_031386.2). This molecular weight is routinely observed by other sources, and was successfully blocked by incubation with the immunising pe

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Flow Cytometry: Flow cytometric analysis showing the expression of NKG2D on a subset of CD56⁺CD3^- NK cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for KLRK1

Target: KLRK1 (Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))

Alternative Name: KLRK1

Background: KLRK1, NKG2-D, killer cell lectin-like receptor subfamily K, member 1, HGNC:18788, D12S2489E, KLR, NK cell receptor D, NKG2-D type II integral membrane protein, CD314, D12S2489E, FLJ17759, FLJ75772, NKG2D

Gene ID: 22914

NCBI Accession: NP_031386

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process