Complement Factor H antibody (Internal Region)

Catalog No. ABIN185437

This Goat Polyclonal antibody specifically detects Complement Factor H in WB, IHC, ELISA and FACS. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for Complement Factor H antibody (Internal Region) (ABIN185437)

Target: Complement Factor H (CFH)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Complement Factor H antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-Complement Factor H Antibody

Binding Specificity: Internal Region

Purpose: Complement factor H

Sequence: HLVPDRKKDQ YK

Specificity: This antibody is expected to recognize isoform a (NP_000177.2) only.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HLVPDRKKDQYK, from the internal region of the protein sequence according to NP_000177.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Liver. Recommended concentration: 5 μg/mL.

Western Blot: Approx 150 kDa band observed in Human Kidney lysates and in lysates of cell line K562 (calculated MW of 139 kDa according to NP_000177.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.03-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-Complement Factor H antibody (ABIN185437)

Fujisawa, Yasumoto, Kato, Sugawara, Yoshida, Yatomi, Nangaku: "The role of anti-complement factor H antibodies in the development of atypical haemolytic uremic syndrome: a possible contribution to abnormality of platelet function." in: British journal of haematology, Vol. 189, Issue 1, pp. 182-186, (2020) (PubMed).

Target Details for Complement Factor H

Target: Complement Factor H (CFH)

Alternative Name: CFH

Background: Complement factor H , HGNC:4883, CFH, CFHL3, FHL1, HF, HF1, HF2, HUS, H factor 1 (complement), H factor 2 (complement), H factor-1 (complement), factor H-like 1, AHUS1, ARMD4, ARMS1, FH, age-related maculopathy susceptibility 1, beta-1-H-globulin, beta-1H

Gene ID: 3075

NCBI Accession: NP_000177

Pathways: Complement System, Cellular Response to Molecule of Bacterial Origin