OCT4 antibody (C-Term)

Catalog No. ABIN185438

The Goat Polyclonal anti-OCT4 antibody has been validated for WB and ELISA. It is suitable to detect OCT4 in samples from Human and Mouse.

Quick Overview for OCT4 antibody (C-Term) (ABIN185438)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This OCT4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-OCT4 Antibody

Binding Specificity: C-Term

Purpose: OCT4 / POU5F1

Sequence: C-VTTLGSPMHS N

Specificity: This antibody is expected to recognise both reported isoforms (NP_002692.2 and NP_976034.3)

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-VTTLGSPMHSN, from the C Terminus of the protein sequence according to NP_002692.2, NP_976034.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 30 kDa band observed in Human Placenta, and approx 28 kDa in Mouse Heart lysates (calculated MW of 30.1 kDa according to Human NP_976034.3 and 25.0 kDa according to Mouse NP_001239381.1). Recommended concentration: 0.5-2 μg/mL. Primary in

Peptide ELISA: antibody detection limit dilution 1:2000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Alternative Name: POU5F1

Background: POU5F1, OTF4, POU domain, class 5, transcription factor 1, HGNC:9221, MGC22487, OCT3, OTF3, POU-type homeodomain-containing DNA-binding protein, octamer-binding transcription factor-3, POU class 5 homeobox 1, DADB-104B20.2, OCT4, POU domain, class 5, tran

Gene ID: 5460, 18999, 294562

NCBI Accession: NP_002692, NP_976034

Pathways: Stem Cell Maintenance