RHPN2 antibody (Internal Region)

Catalog No. ABIN185439

This anti-RHPN2 antibody is a Goat Polyclonal antibody detecting RHPN2 in ELISA and IHC. Suitable for Human.

Quick Overview for RHPN2 antibody (Internal Region) (ABIN185439)

Target: RHPN2 (Rhophilin, rho GTPase Binding Protein 2 (RHPN2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This RHPN2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-RHPN2 Antibody

Binding Specificity: Internal Region

Purpose: Rhophilin 2 (Internal)

Sequence: TNKNRQKSAS T

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TNKNRQKSAST, from the internal region of the protein sequence according to NP_149094.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Kidney shows staining of cellular membrane and cytoplasm in convoluted tubular cells. Recommended concentration 5-10 μg/mL.

Western Blot: Preliminary experiments gave an approx 35 kDa band in Human Pancreas and Prostate lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculat

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RHPN2

Target: RHPN2 (Rhophilin, rho GTPase Binding Protein 2 (RHPN2))

Alternative Name: RHPN2

Background: RHPN2, p76RBE, rhophilin, Rho GTPase binding protein 2, rhophilin 2, rhophilin-2, RhoB effector, rhophilin-like protein, P76RBE, RHOBP, 76 kDa RhoB effector protein, GTP-Rho-binding protein 2, rhophilin-like Rho-GTPase binding protein

Gene ID: 85415

NCBI Accession: NP_149094