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Adenosine A2a Receptor antibody (C-Term)

The Goat Polyclonal anti-Adenosine A2a Receptor antibody has been validated for WB, ELISA and IF. It is suitable to detect Adenosine A2a Receptor in samples from Human, Mouse and Macaque. There is 1 publication available.
Catalog No. ABIN185549

Quick Overview for Adenosine A2a Receptor antibody (C-Term) (ABIN185549)

Target

See all Adenosine A2a Receptor (ADORA2A) Antibodies
Adenosine A2a Receptor (ADORA2A)

Reactivity

  • 81
  • 29
  • 27
  • 9
  • 6
  • 4
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
Human, Mouse, Macaque

Host

  • 71
  • 12
  • 1
  • 1
Goat

Clonality

  • 74
  • 10
  • 1
Polyclonal

Conjugate

  • 53
  • 6
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Adenosine A2a Receptor antibody is un-conjugated

Application

  • 64
  • 30
  • 24
  • 20
  • 14
  • 12
  • 9
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade

Verified
  • Binding Specificity

    • 12
    • 8
    • 7
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Purpose

    Adenosine A2a Receptor

    Sequence

    HELKGVCPEP PGLDD

    Cross-Reactivity

    Human

    Purification

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    Immunogen

    Peptide with sequence HELKGVCPEPPGLDD, from the C Terminus of the protein sequence according to NP_000666.2.

    Isotype

    IgG
  • Application Notes

    Western Blot: This antibody has been successfully used in Western blot on Macaque: van der Putten C et al. (2009) PMID: 19494284. This antibody has been successfully used in Western blot on Mouse: He X et al. (2013) PMID: 23712033, and Zhao L et al. (201

    Peptide ELISA: antibody detection limit dilution 1:8000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 10μg/ml.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.5 mg/mL

    Buffer

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Minimize freezing and thawing.

    Storage

    -20 °C

    Storage Comment

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • van der Putten, Zuiderwijk-Sick, van Straalen, de Geus, Boven, Kondova, IJzerman, Bajramovic: "Differential expression of adenosine A3 receptors controls adenosine A2A receptor-mediated inhibition of TLR responses in microglia." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 12, pp. 7603-12, (2009) (PubMed).

  • Target

    Adenosine A2a Receptor (ADORA2A)

    Alternative Name

    ADORA2A

    Background

    ADORA2A, adenosine A2a receptor , HGNC:263, ADORA2, RDC8, hA2aR , adenosine A2 receptor, adenosine receptor subtype A2a

    Gene ID

    135

    NCBI Accession

    NP_000666

    Pathways

    Neurotrophin Signaling Pathway, cAMP Metabolic Process, Synaptic Membrane, Feeding Behaviour, Cancer Immune Checkpoints
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