Adenosine A2a Receptor antibody (C-Term)
Quick Overview for Adenosine A2a Receptor antibody (C-Term) (ABIN185549)
Target
See all Adenosine A2a Receptor (ADORA2A) AntibodiesReactivity
Host
Clonality
Conjugate
Application
Grade
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Binding Specificity
- C-Term
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Purpose
- Adenosine A2a Receptor
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Sequence
- HELKGVCPEP PGLDD
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Cross-Reactivity
- Human
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Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Immunogen
- Peptide with sequence HELKGVCPEPPGLDD, from the C Terminus of the protein sequence according to NP_000666.2.
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Isotype
- IgG
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Application Notes
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Western Blot: This antibody has been successfully used in Western blot on Macaque: van der Putten C et al. (2009) PMID: 19494284. This antibody has been successfully used in Western blot on Mouse: He X et al. (2013) PMID: 23712033, and Zhao L et al. (201
Peptide ELISA: antibody detection limit dilution 1:8000.
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Comment
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 10μg/ml.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Minimize freezing and thawing.
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Storage
- -20 °C
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Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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Differential expression of adenosine A3 receptors controls adenosine A2A receptor-mediated inhibition of TLR responses in microglia." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 12, pp. 7603-12, (2009) (PubMed).
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Differential expression of adenosine A3 receptors controls adenosine A2A receptor-mediated inhibition of TLR responses in microglia." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 12, pp. 7603-12, (2009) (PubMed).
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- Adenosine A2a Receptor (ADORA2A)
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Alternative Name
- ADORA2A
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Background
- ADORA2A, adenosine A2a receptor , HGNC:263, ADORA2, RDC8, hA2aR , adenosine A2 receptor, adenosine receptor subtype A2a
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Gene ID
- 135
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NCBI Accession
- NP_000666
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Pathways
- Neurotrophin Signaling Pathway, cAMP Metabolic Process, Synaptic Membrane, Feeding Behaviour, Cancer Immune Checkpoints
Target
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