Chromogranin A antibody (Internal Region)

Catalog No. ABIN185645

The Goat Polyclonal anti-Chromogranin A antibody has been validated for FACS, WB and ELISA. It is suitable to detect Chromogranin A in samples from Human.

Quick Overview for Chromogranin A antibody (Internal Region) (ABIN185645)

Target: Chromogranin A (CHGA)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Chromogranin A antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-Chromogranin A Antibody

Binding Specificity: Internal Region

Purpose: Chromogranin A precursor

Sequence: QGAKERAHQQ KKH

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QGAKERAHQQKKH, from the internal region of the protein sequence according to NP_001266.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 50 kDa band observed in lysates of cell lines Jurkat and LNCaP, and in Human Cerebellum, Pancreas and Kidney lysates. A band at 35 kDa was also observed in pancreas lysate (calculated MW of 50.7 kDa according to NP_001266.1 isoform 1 a

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Chromogranin A

Target: Chromogranin A (CHGA)

Alternative Name: CHGA

Background: Chromogranin A precursor, chromogranin A (parathyroid secretory protein 1) , CHGA, CGA , parathyroid secretory protein 1, Vasostatin, Pancreastatin, Parastatin, Pituitary secretory protein I, SP1

Gene ID: 1113

NCBI Accession: NP_001266, NP_001288619

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling