Septin 1 antibody (Internal Region)

Catalog No. ABIN185707

This Goat Polyclonal antibody specifically detects Septin 1 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for Septin 1 antibody (Internal Region) (ABIN185707)

Target: Septin 1 (SEPT1)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Septin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-Septin 1 Antibody

Binding Specificity: Internal Region

Purpose: Septin 1 / SEPT1

Sequence: GARDRASRSK LSRQ

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-GARDRASRSKLSRQ, from the internal region of the protein sequence according to NP_443070.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 6-8 μg/mL.

Western Blot: Approx 42-45 kDa band observed in lysates of cell lines HEK293 and MOLT-4 (calculated MW of 42.4 kDa according to NP_443070.6). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour. at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Septin 1

Target: Septin 1 (SEPT1)

Alternative Name: SEPT1

Background: SEPT1, septin 1 , DIFF6, LARP, MGC20394, PNUTL3, SEP1 , differentiation 6 (deoxyguanosine triphosphate triphosphohydrolase), serologically defined breast cancer antigen NY-BR-24

Gene ID: 1731, 54204, 293507

NCBI Accession: NP_443070