Ghrelin antibody (AA 25-117)

Catalog No. ABIN1859007

This anti-Ghrelin antibody is a Rabbit Polyclonal antibody detecting Ghrelin in WB, IHC, IP and ICC. Suitable for Human.

Quick Overview for Ghrelin antibody (AA 25-117) (ABIN1859007)

Target: Ghrelin (GHRL)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ghrelin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Ghrelin Antibody

Binding Specificity: AA 25-117

Purpose: Polyclonal Antibody to Ghrelin/Obestatin Preprohormone (GHRL)

Sequence: MGHHHHHHSG SEF- SSFLSP EHQRVQQRKE SKKPPAKLQP RALAGWLRPE DGGQAEGAED ELEVRFNAPF DVGIKLSGVQ YQQHSQALGK FLQDILWEEA KEAPADK

Specificity: The antibody is a rabbit polyclonal antibody raised against GHRL. It has been selected for its ability to recognize GHRL in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPD252Hu01Recombinant Ghrelin/Obestatin Preprohormone (GHRL)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Ghrelin

Target: Ghrelin (GHRL)

Alternative Name: GHRL

Background: MTLRP, Preproghrelin, Growth Hormone Secretagogue Receptor Ligand, Appetite-regulating hormone, Growth hormone-releasing peptide, Motilin-related peptide

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Peptide Hormone Metabolism, Negative Regulation of Hormone Secretion, Synaptic Membrane, Feeding Behaviour