P4HB antibody (AA 216-465)

Catalog No. ABIN1860168

This anti-P4HB antibody is a Rabbit Polyclonal antibody detecting P4HB in WB. Suitable for Human.

Quick Overview for P4HB antibody (AA 216-465) (ABIN1860168)

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P4HB antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-P4HB Antibody

Binding Specificity: AA 216-465

Purpose: Polyclonal Antibody to Protein Disulfide Isomerase (PDI)

Specificity: The antibody is a rabbit polyclonal antibody raised against PDI. It has been selected for its ability to recognize PDI in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPB061Hu01Recombinant Protein Disulfide Isomerase (PDI)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.27 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for P4HB

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Alternative Name: Protein Disulfide Isomerase

Background: P4HB, ERBA2L, PDIA1, PO4DB, PROHB, DSI, GIT, PDI, PO4HB, P4Hbeta, Prolyl 4-Hydroxylase Subunit Beta, Procollagen-Proline 2-Oxoglutarate 4-Dioxygenase Beta Polypeptide

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism