TIMP1 antibody (AA 24-207) (APC)

Catalog No. ABIN1860758

The Guinea Pig Polyclonal anti-TIMP1 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect TIMP1 in samples from Rabbit.

Quick Overview for TIMP1 antibody (AA 24-207) (APC) (ABIN1860758)

Target: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Reactivity: Rabbit

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This TIMP1 antibody is conjugated to APC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-TIMP1 Antibody (APC)

Binding Specificity: AA 24-207

Purpose: Polyclonal Antibody to Tissue Inhibitors Of Metalloproteinase 1 (TIMP1)

Sequence: MGHHHHHHSG SEF-CTCVPPH PQTAFCNSDL VIRAKFVGAP EVNHTTLYQR YEIKTTKMFK GFDALGHATD IRFVYTPAME SVCGYSHKSQ NRSEEFLIAG QLRNGLLHIT TCSFVVPWNS LSFSQRSGFT KTYAAGCDMC TVFACASIPC HLESDTHCLW TDQLLLGSDK GFQSRHLACL PQEPGLCAWQ SLRPRKD

Specificity: The antibody is a cavia polyclonal antibody raised against TIMP1. It has been selected for its ability to recognize TIMP1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: TIMP1 (AA 24-207)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for TIMP1

Target: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Alternative Name: TIMP1

Background: EPO, CLGI, EPA, HCI, Erythroid Potentiating Activity, Collagenase Inhibitor, Metalloproteinase inhibitor 1, Fibroblast collagenase inhibitor