S100A12 antibody

Catalog No. ABIN1865888

The Mouse Monoclonal anti-S100A12 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect S100A12 in samples from Human.

Quick Overview for S100A12 antibody (ABIN1865888)

Target: S100A12 (S100 Calcium Binding Protein A12 (S100A12))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100A12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 15#

Product Details anti-S100A12 Antibody

Purpose: Monoclonal Antibody to S100 Calcium Binding Protein A12 (S100A12)

Specificity: The antibody is a mouse monoclonal antibody raised against S100A12. It has been selected for its ability to recognize S100A12 in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: RPB080Hu01Recombinant S100 Calcium Binding Protein A12 (S100A12)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for S100A12

Target: S100A12 (S100 Calcium Binding Protein A12 (S100A12))

Alternative Name: S100A12

Background: CGRP, P6, CAAF1, CAGC, ENRAGE, MRP6, Calgranulin C, Calcium-binding protein in amniotic fluid 1, Extracellular newly identified RAGE-binding, Migration inhibitory factor-related 6

Pathways: Toll-Like Receptors Cascades, S100 Proteins