Cholesterol antibody (Biotin)

Catalog No. ABIN1872491

The Rabbit Polyclonal anti-Cholesterol antibody has been validated for IP, ICC and IHC. It is suitable to detect Cholesterol in samples from Various Species.

Quick Overview for Cholesterol antibody (Biotin) (ABIN1872491)

Target: Cholesterol (CH)

Reactivity: Various Species

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cholesterol antibody is conjugated to Biotin

Application: Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Product Details anti-Cholesterol Antibody (Biotin)

Purpose: Biotin-Linked Polyclonal Antibody to Cholesterol (CH)

Specificity: The antibody is a rabbit polyclonal antibody raised against CH. It has been selected for its ability to recognize CH in immunohistochemical staining and western blotting.

Characteristics: Chemical Formula: C27H46O
Molecular Mass: 386.65 g/mol

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: PAB701Ge01Polyclonal Antibody to Cholesterol (CH)

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry: 5-20 μg/mL Immunocytochemistry: 5-20 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Reconstitute in sterile PBS, pH 7.2 - pH 7.4.

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Cholesterol

Target: Cholesterol (CH)

Alternative Name: Cholesterol

Target Type: Hormone