FASL antibody

Catalog No. ABIN187289

The Rat Monoclonal anti-FASL antibody has been validated for WB, FACS and IF. It is suitable to detect FASL in samples from Mouse.

Quick Overview for FASL antibody (ABIN187289)

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This FASL antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: H11

Product Details anti-FASL Antibody

Specificity: Species Reactivity: Mouse. Others not tested.

Purity: > 95 % by SDS-PAGE.

Immunogen: Synthetic peptide corresponding to aa 196-220 of the mouse Fas Ligand protein (CD95L, APO-1L, CD178).

Isotype: IgG2a

Application Details

Application Notes: Immunocytochemistry: Use anti-Fas Ligand, clone H11 unlabeled (Cat. No. MC-106), biotin-labeled (Cat. No. MC-126), or FITC-labeled (Cat. No. MC-136). Flow Cytometry: Use Anti-Fas Ligand, clone H11 unlabeled (Cat. No. MC-106), biotin-labeled (Cat. No. MC-126), or FITC-labeled (Cat. No. MC-136). [ Note: Activated T cells do not express high levels of surface Fas Ligand.] Western Blot: Excellent for this application. Use 2 µg/mL. Use unlabeled (Cat. No. MC-106) or biotin-labeled (Cat. No. MC-126). The optimal dilution for a specific application should be determined by the researcher. Figure 1. Immunocytochemistry: Detection of FasL in 293T cells transfected with a FasL expression vector. Method: Transfected cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20 °C for 5 min, then in acetone at -20 °C for 30 sec. After 3 washes in PBS, 0.1% BSA, slides were incubated for 1 hr at RT with 20 µg/mL of biotinylated H11 antibody (MC-126) in PBS, 0.1% BSA. After rinsing in PBS, FITC-conjugated streptavidin was added for 30 min. Slides were washed again in PBS and visualized using a fluorescence microscope. Method: FasL transfected 293T cells (5x105) were incubated on ice for 30 min in 50 µL FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1 µg of FITC-labeled H11 antibody (MC-136). After washing in FACS buffer, cells were analyzed by flow cytometry. Do not exclude pre-apoptotic cells during data acquisition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 µL of 1 mg/mL antibody in PBS with 0.02% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for FASL

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Alternative Name: CD178 / Fas Ligand

Background: Fas Ligand (FasL) is a type II membrane protein and a member of the tumor necrosis factor family. FasL is released by cleavage by metalloproteinases. When interacting with the Fas receptor, the trimeric FasL induces apoptosis. It is responsible for the activation-induced cell death of peripheral T cells and functions as an effector molecule for cytotoxic T cells. FasL is expressed in activated lymphocytes, Sertoli cells, and in certain tumor cells.

Pathways: Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity