UCHL5 antibody (AA 136-164)

Catalog No. ABIN1881974

The Rabbit Polyclonal anti-UCHL5 antibody has been validated for WB. It is suitable to detect UCHL5 in samples from Human. There are 5+ publications available.

Quick Overview for UCHL5 antibody (AA 136-164) (ABIN1881974)

Target: UCHL5 (Ubiquitin Carboxyl-terminal Hydrolase L5 (UCHL5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCHL5 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB42329

Product Details anti-UCHL5 Antibody

Binding Specificity: AA 136-164

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This UCHL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 136-164 amino acids from the Central region of human UCHL5.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-UCHL5 antibody (ABIN1881974)

Nishio, Kim, Kawai, Mizushima, Yamane, Hamazaki, Murata, Tanaka, Morimoto: "Crystal structure of the de-ubiquitinating enzyme UCH37 (human UCH-L5) catalytic domain." in: Biochemical and biophysical research communications, Vol. 390, Issue 3, pp. 855-60, (2009) (PubMed).

Yao, Song, Jin, Cai, Takahashi, Swanson, Washburn, Florens, Conaway, Cohen, Conaway: "Distinct modes of regulation of the Uch37 deubiquitinating enzyme in the proteasome and in the Ino80 chromatin-remodeling complex." in: Molecular cell, Vol. 31, Issue 6, pp. 909-17, (2008) (PubMed).

Koulich, Li, DeMartino: "Relative structural and functional roles of multiple deubiquitylating proteins associated with mammalian 26S proteasome." in: Molecular biology of the cell, Vol. 19, Issue 3, pp. 1072-82, (2008) (PubMed).

Hamazaki, Iemura, Natsume, Yashiroda, Tanaka, Murata: "A novel proteasome interacting protein recruits the deubiquitinating enzyme UCH37 to 26S proteasomes." in: The EMBO journal, Vol. 25, Issue 19, pp. 4524-36, (2006) (PubMed).

Qiu, Ouyang, Li, Miao, Wang, Goldberg: "hRpn13/ADRM1/GP110 is a novel proteasome subunit that binds the deubiquitinating enzyme, UCH37." in: The EMBO journal, Vol. 25, Issue 24, pp. 5742-53, (2006) (PubMed).

Target Details for UCHL5

Target: UCHL5 (Ubiquitin Carboxyl-terminal Hydrolase L5 (UCHL5))

Alternative Name: UCHL5

Background: Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome.

Molecular Weight: 37607

NCBI Accession: NP_001186190, NP_001186191, NP_001186192, NP_057068

UniProt: Q9Y5K5