PIAS3 antibody (N-Term)

Catalog No. ABIN1882116

Product Details anti-PIAS3 Antibody

Target: PIAS3 (Protein Inhibitor of Activated STAT, 3 (PIAS3))

Binding Specificity: AA 95-126, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIAS3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: M, Rat

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This PIAS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-126 amino acids from the N-terminal region of human PIAS3.

Clone: RB0671

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-PIAS3 antibody (ABIN1882116)

Spoden, Morandell, Ehehalt, Fiedler, Jansen-Dürr, Hermann, Zwerschke: "The SUMO-E3 ligase PIAS3 targets pyruvate kinase M2." in: Journal of cellular biochemistry, Vol. 107, Issue 2, pp. 293-302, (2009) (PubMed).

Brantley, Nabors, Gillespie, Choi, Palmer, Harrison, Roarty, Benveniste: "Loss of protein inhibitors of activated STAT-3 expression in glioblastoma multiforme tumors: implications for STAT-3 activation and gene expression." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 15, pp. 4694-704, (2008) (PubMed).

Iwasaki, Hailemariam, Tsuji: "PIAS3 interacts with ATF1 and regulates the human ferritin H gene through an antioxidant-responsive element." in: The Journal of biological chemistry, Vol. 282, Issue 31, pp. 22335-43, (2007) (PubMed).

Nojiri, Joh, Miura, Sakata, Nomura, Nakao, Sobue, Ohara, Asai, Ito: "ATBF1 enhances the suppression of STAT3 signaling by interaction with PIAS3." in: Biochemical and biophysical research communications, Vol. 314, Issue 1, pp. 97-103, (2004) (PubMed).

Long, Wang, Matsuura, He, Liu: "Activation of Smad transcriptional activity by protein inhibitor of activated STAT3 (PIAS3)." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 1, pp. 99-104, (2004) (PubMed).

Cheng, Zhang, Zhou, Marasco: "Down-regulation of SHP1 and up-regulation of negative regulators of JAK/STAT signaling in HTLV-1 transformed cell lines and freshly transformed human peripheral blood CD4+ T-cells." in: Leukemia research, Vol. 28, Issue 1, pp. 71-82, (2003) (PubMed).

Yamamoto, Sato, Sekine, Yumioka, Imoto, Junicho, Fuse, Matsuda: "Molecular interactions between STAT3 and protein inhibitor of activated STAT3, and androgen receptor." in: Biochemical and biophysical research communications, Vol. 306, Issue 2, pp. 610-5, (2003) (PubMed).

Ueki, Seki, Yano, Saito, Masuho, Muramatsu: "Isolation and chromosomal assignment of a human gene encoding protein inhibitor of activated STAT3 (PIAS3)." in: Journal of human genetics, Vol. 44, Issue 3, pp. 193-6, (1999) (PubMed).

Target Details for PIAS3

Target: PIAS3 (Protein Inhibitor of Activated STAT, 3 (PIAS3))

Alternative Name: PIAS3 (PIAS3 Products)

Background: PIAS3 is a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor, catalyzing the covalent attachment of a SUMO protein to specific target substrates. PIAS3 plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context.

Molecular Weight: 68017

NCBI Accession: NP_006090

UniProt: Q9Y6X2

Pathways: JAK-STAT Signaling