USP14 antibody

Catalog No. ABIN1882288

This Mouse Monoclonal antibody specifically detects USP14 in WB. It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 3+ publications.

Quick Overview for USP14 antibody (ABIN1882288)

Target: USP14 (Ubiquitin Specific Peptidase 14 (USP14))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This USP14 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 889CT6-2-1

Product Details anti-USP14 Antibody

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: Purified His-tagged USP14 protein was used to produced this monoclonal antibody.

Isotype: IgG1

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-USP14 antibody (ABIN1882288)

Carrascal, Ovelleiro, Casas, Gay, Abian: "Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment." in: Journal of proteome research, Vol. 7, Issue 12, pp. 5167-76, (2009) (PubMed).

Koulich, Li, DeMartino: "Relative structural and functional roles of multiple deubiquitylating proteins associated with mammalian 26S proteasome." in: Molecular biology of the cell, Vol. 19, Issue 3, pp. 1072-82, (2008) (PubMed).

Reuter, Medhurst, Waisfisz, Zhi, Herterich, Hoehn, Gross, Joenje, Hoatlin, Mathew, Huber: "Yeast two-hybrid screens imply involvement of Fanconi anemia proteins in transcription regulation, cell signaling, oxidative metabolism, and cellular transport." in: Experimental cell research, Vol. 289, Issue 2, pp. 211-21, (2003) (PubMed).

Target Details for USP14

Target: USP14 (Ubiquitin Specific Peptidase 14 (USP14))

Alternative Name: USP14

Background: Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs).

Molecular Weight: 56069

NCBI Accession: NP_001032411, NP_005142

UniProt: P54578