P4HB antibody (AA 23-197)

Catalog No. ABIN1886225

The Rabbit Polyclonal anti-P4HB antibody has been validated for WB, IHC and IF. It is suitable to detect P4HB in samples from Human.

Quick Overview for P4HB antibody (AA 23-197) (ABIN1886225)

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P4HB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-P4HB Antibody

Binding Specificity: AA 23-197

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 23 and 197 of Human P4HB

Application Details

Application Notes: Suggested dilutions:
Western blotting: 1.500-1.3000
Immunohistochemistry: 1.100-1.250
Immunofluorescence: 1.100-1.200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.1 M Tris-buffered saline with 10 % Glycerol (pH 7.0).0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: Biohazard Informations: This product contains thimerosal which is hazardous.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for P4HB

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Alternative Name: PDI

Background: This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family.When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen.This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds.Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.[provided by RefSeq]

Molecular Weight: 57 kDa

Gene ID: 5034

NCBI Accession: NP_000909, NM_000918

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism