Rubisco antibody

Catalog No. ABIN190723

This Rabbit Polyclonal antibody specifically detects Rubisco in WB. It exhibits reactivity toward Arabidopsis thaliana, Spinach, Chlamydomonas, Potato, Soybean and Synechocystis PCC 6803 and has been mentioned in 2+ publications.

Quick Overview for Rubisco antibody (ABIN190723)

Target: Rubisco (RuBisCO) (Ribulose-1,5-Bisphosphate Carboxylase/oxygenase (RuBisCO))

Reactivity: Arabidopsis thaliana, Spinach, Chlamydomonas, Potato, Soybean, Synechocystis PCC 6803

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Rubisco antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Rubisco Antibody

Characteristics: Expected / apparent Molecular Weight of the Antigene: 53-55 / 53-55 kDa

Purification: serum

Immunogen: purified 557 kDa hexadecamer Rubisco protein complex from Spinacia oleracea (SIGMA-ALDRICH R-8000)

Application Details

Application Notes: Recommended Dilution: 1 : 10 000 - 1 : 20 000 on 0.5-10 ug total cellular protein/lane and standard ECL(WB).

Comment:

RbcS subunit is not detected by this antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 µL of sterile water.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

References for anti-Rubisco antibody (ABIN190723)

Kissen, Hyldbakk, Wang, Sørmo, Rossiter, Bones: "Ecotype dependent expression and alternative splicing of epithiospecifier protein (ESP) in Arabidopsis thaliana." in: Plant molecular biology, Vol. 78, Issue 4-5, pp. 361-75, (2012) (PubMed).

Li, Zhu, Zeng, Zhang, Ye, Ou, Rehman, Heuer, Chen: "Proteome characterization of cassava (Manihot esculenta Crantz) somatic embryos, plantlets and tuberous roots." in: Proteome science, Vol. 8, pp. 10, (2010) (PubMed).

Target Details for Rubisco

Target: Rubisco (RuBisCO) (Ribulose-1,5-Bisphosphate Carboxylase/oxygenase (RuBisCO))

Alternative Name: Rubisco

Background: Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.

Molecular Weight: expected: 53-55 kDa, apparent: 53-55 kDa

UniProt: Q43832, P00875