TACR1 antibody (Internal Region)

Catalog No. ABIN190795

This Goat Polyclonal antibody specifically detects TACR1 in WB, ELISA and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for TACR1 antibody (Internal Region) (ABIN190795)

Target: TACR1 (Tachykinin Receptor 1 (TACR1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This TACR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-TACR1 Antibody

Binding Specificity: Internal Region

Purpose: TACR1

Sequence: EIPGDSSDRY HEQ

Specificity: This antibody is expected to recognise both reported isoforms (NP_001049.1 and NP_056542.1).

Cross-Reactivity: Dog, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EIPGDSSDRYHEQ, from the internal region of the protein sequence according to NP_001049.1*, NP_056542.1*.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 48 kDa band observed in lysates of cell lines HeLa and Neuro2a (calculated MW of 46.3 kDa according to Human NP_001049.1 and Mouse NP_033339.2 ). Recommended concentration: 0.3-1 μg/mL.. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Expression of the protein seen in the plasma membrane and nucleus of Neuro2a cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TACR1

Target: TACR1 (Tachykinin Receptor 1 (TACR1))

Alternative Name: TACR1

Background: TACR1, tachykinin receptor 1, NK1R, neurokinin 1 receptor, NKIR, SPR, TAC1R , NK-1 receptor, Tachykinin receptor 1 (substance P receptor,neurokinin-1 receptor), neurokinin 1 receptor, tachykinin 1 receptor (substance P receptor, neurokinin 1 receptor)

Gene ID: 6869, 21336, 24807

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration