ASS1 antibody (Internal Region)

Catalog No. ABIN190834

This Goat Polyclonal antibody specifically detects ASS1 in WB, ELISA, IHC, FACS and IF. It exhibits reactivity toward Human, Mouse and Ratand has been mentioned in 1 publication.

Quick Overview for ASS1 antibody (Internal Region) (ABIN190834)

Target: ASS1 (Argininosuccinate Synthase 1 (ASS1))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This ASS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-ASS1 Antibody

Binding Specificity: Internal Region

Purpose: Argininosuccinate synthetase 1

Sequence: ENPKNQAPPG LYTKTQD

Specificity: The variants represent identical protein (NP_000041.2 and NP_446464.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-ENPKNQAPPGLYTKTQD

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Kidney shows textured cytoplasm staining in PCT. Recommended concentration: 2-4 μg/mL. Paraffin embedded Human Liver. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 45 kDa band observed in lysates of cell lines A431 and NIH3T3 and approx.48 kDa in Human and Rat Kidney and in Mouse Liver lysates (calculated MW of 46.5 kDa according to Human NP_000041.2 and Rat NP_037289.1, and 46.6 kDa according to

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.<

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-ASS1 antibody (ABIN190834)

Corbin, Pendleton, Solomonson, Eichler: "Phosphorylation of argininosuccinate synthase by protein kinase A." in: Biochemical and biophysical research communications, Vol. 377, Issue 4, pp. 1042-6, (2008) (PubMed).

Target Details for ASS1

Target: ASS1 (Argininosuccinate Synthase 1 (ASS1))

Alternative Name: ASS1

Background: Argininosuccinate synthetase 1, ASS, ASS1, CTLN1, citrulline--aspartate ligase

Gene ID: 445, 11898, 25698

NCBI Accession: NP_000041, NP_446464

Pathways: Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin