Nanog antibody (C-Term)

Catalog No. ABIN190835

The Goat Polyclonal anti-Nanog antibody has been validated for WB, ELISA and IHC. It is suitable to detect Nanog in samples from Human.

Quick Overview for Nanog antibody (C-Term) (ABIN190835)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Nanog antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-Nanog Antibody

Binding Specificity: C-Term

Purpose: NANOG (C Terminus)

Sequence: DLFLNYSMNM QPED

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DLFLNYSMNMQPED, from the C Terminus of the protein sequence according to NP_079141.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Testis shows nuclear staining in spermatogonia. Recommended concentration, 1-2 μg/mL.

Western Blot: Approx 35 kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 34.6 kDa according to NP_079141.1). Recommended concentration: 0.05-0.1 μg/mL. An additional band of approx. 37 kDa is observed and is consistent with the obser

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Alternative Name: NANOG

Background: NANOG, Nanog homeobox, homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48

Gene ID: 79923

NCBI Accession: NP_079141

Pathways: Stem Cell Maintenance