NCDN antibody (Internal Region)

Catalog No. ABIN190873

This Goat Polyclonal antibody specifically detects NCDN in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for NCDN antibody (Internal Region) (ABIN190873)

Target: NCDN (Neurochondrin (NCDN))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NCDN antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-NCDN Antibody

Binding Specificity: Internal Region

Purpose: Neurochondrin

Sequence: HYLLQVGSEK QKEP

Specificity: This antibody is expected to recognize both reported isoforms (NP_055099.1, NP_001014841.1). Reported variants represent identical protein (NP_055099.1, NP_001014839.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HYLLQVGSEKQKEP, from the internal region of the protein sequence according to NP_055099.1, NP_001014841.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 75 kDa band observed in lysates of cell lines HepG2 and U251 (calculated MW of 77.2 kDa according to NP_001014841.1). An additional band of unknown identity was also observed in U251 lysates at 55 kDa. This band was successfully blocke

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NCDN

Target: NCDN (Neurochondrin (NCDN))

Alternative Name: NCDN

Background: NCDN, neurochondrin , KIAA0607

Gene ID: 23154, 26562, 89791

NCBI Accession: NP_055099, NP_001014841