Laminin 5 antibody

Catalog No. ABIN191945

This Mouse Monoclonal antibody specifically detects Laminin 5 in WB, IHC (fro) and EIA. It exhibits reactivity toward Human and Rat and has been mentioned in 4+ publications.

Quick Overview for Laminin 5 antibody (ABIN191945)

Target: Laminin 5 (LN-5)

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Laminin 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 6F12

Product Details anti-Laminin 5 Antibody

Characteristics: Synonyms: Laminin-5

Purification: Protein A Chromatography

Immunogen: Human Laminin 5 beta 3 chain

Isotype: IgG

Application Details

Application Notes: Western blot. ELISA (coating antibody in Sandwich ELISA). Immunohistochemistry on Cryosections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in aqua bidest to initial volume.

Buffer: PBS, pH 7.4

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized at 2-8 °C and reconstituted at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

References for anti-Laminin 5 antibody (ABIN191945)

Drumea-Mirancea, Wessels, Müller, Essl, Eble, Tolosa, Koch, Reinhardt, Sixt, Sorokin, Stierhof, Schwarz, Klein: "Characterization of a conduit system containing laminin-5 in the human thymus: a potential transport system for small molecules." in: Journal of cell science, Vol. 119, Issue Pt 7, pp. 1396-405, (2006) (PubMed).

Amano, Akutsu, Ogura, Nishiyama: "Increase of laminin 5 synthesis in human keratinocytes by acute wound fluid, inflammatory cytokines and growth factors, and lysophospholipids." in: The British journal of dermatology, Vol. 151, Issue 5, pp. 961-70, (2004) (PubMed).

Sasaki, Amano, Nishiyama, Nakayama, Burgeson, Nakajima: "Expression of laminin 5, and collagen IV and VII in bullous pemphigoid skin." in: Archives of dermatological research, Vol. 290, Issue 5, pp. 283-5, (1998) (PubMed).

Barnstable, Bodmer, Brown, Galfre, Milstein, Williams, Ziegler: "Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis." in: Cell, Vol. 14, Issue 1, pp. 9-20, (1978) (PubMed).

Target Details for Laminin 5

Target: Laminin 5 (LN-5)

Alternative Name: Laminin 5

Background: Laminins are basement membrane components thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. laminin 5 is a complex glycoprotein composed of three subunits (alpha, beta, and gamma). Laminin 5 is thought to be involved in cell adhesion, signal transduction and differentiation of keratinocytes. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa. Alternatively spliced transcript variants encoding different isoforms have been identified.Synonyms: Laminin-5