IgE antibody (PE)
Quick Overview for IgE antibody (PE) (ABIN192257)
Target
See all IgE AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Purpose
- Anti-Hu IgE PE
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Specificity
- The antibody BE5 reacts with human IgE, it recognizes an epitope different from the ones recognized by 4G7.325 and 4H10 antibodies to IgE.
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Cross-Reactivity (Details)
- Human
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Purification
- Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
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Immunogen
- Purified human IgE.
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Isotype
- IgG1
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Application Notes
- Flow cytometry: Recommended dilution: 2-5 μg/mL.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.1 mg/mL
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Buffer
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
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Do not freeze.
Avoid prolonged exposure to light. -
Storage
- 4 °C
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Storage Comment
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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Reduction of cross-reactive carbohydrate determinants in plant foodstuff: elucidation of clinical relevance and implications for allergy diagnosis." in: PLoS ONE, Vol. 6, Issue 3, pp. e17800, (2011) (PubMed).
: "
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Reduction of cross-reactive carbohydrate determinants in plant foodstuff: elucidation of clinical relevance and implications for allergy diagnosis." in: PLoS ONE, Vol. 6, Issue 3, pp. e17800, (2011) (PubMed).
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- IgE
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Background
- Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.,Immunoglobulin E
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Molecular Weight
- 180 kDa
Target
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