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Asparaginase antibody (Biotin)

This anti-Asparaginase antibody is a Rabbit Polyclonal antibody detecting Asparaginase in WB and ELISA. Suitable for E. coli.
Catalog No. ABIN1952592

Quick Overview for Asparaginase antibody (Biotin) (ABIN1952592)

Target

See all Asparaginase (ASPG) Antibodies
Asparaginase (ASPG) (Asparaginase Homolog (ASPG))

Reactivity

  • 9
  • 3
  • 1
  • 1
E. coli

Host

  • 14
Rabbit

Clonality

  • 14
Polyclonal

Conjugate

  • 8
  • 2
  • 1
  • 1
  • 1
  • 1
This Asparaginase antibody is conjugated to Biotin

Application

  • 13
  • 11
  • 2
  • 1
  • 1
Western Blotting (WB), ELISA
  • Specificity

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Asparaginase [E. coli]. Cross reactivity against Asparaginase from other sources may occur but have not been specifically determined.

    Purification

    Ion exchange chromatography

    Immunogen

    Asparaginase [E. coli].

    Type of Immunogen: Purified protein
  • Application Notes

    Approved: ELISA (1:5000 - 1:25000), WB (1:500 - 1:3000)

    Usage: Anti-L-Asparaginase has been assayed against 1.0 μg of Asparaginase in a standard capture ELISA using Peroxidase Conjugated Streptavidin ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:3 to 1:12 of the reconstitution concentration is suggested for this product. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    Comment

    Target Species of Antibody: E. coli

    Restrictions

    For Research Use only
  • Format

    Liquid

    Reconstitution

    Distilled water

    Concentration

    Lot specific

    Buffer

    0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 1 % BSA

    Handling Advice

    avoid freeze thaw cycles

    Storage

    4 °C,-20 °C

    Storage Comment

    Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
  • Target

    Asparaginase (ASPG) (Asparaginase Homolog (ASPG))

    Alternative Name

    Asparaginase
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