Pyruvate Kinase, Muscle (PKM) antibody (Biotin)

Catalog No. ABIN1968075

The Biotin-conjugated Goat Polyclonal anti-Pyruvate Kinase, Muscle antibody (ABIN1968075) specifically detects Pyruvate Kinase, Muscle in WB and ELISA. The antibody is reactive with Rabbit samples.

Quick Overview for Pyruvate Kinase, Muscle (PKM) antibody (Biotin) (ABIN1968075)

Target: Pyruvate Kinase, Muscle (PKM)

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: Biotin

Application: Western Blotting (WB), ELISA

Product Details

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pyruvate Kinase [Rabbit Muscle]. Anti-Pyruvate Kinase has been reported to react with all forms of pyruvate kinase (pan M-PK). Cross reactivity against Pyruvate Kinase from other sources may occur but have not been specifically determined.

Purification: Ion exchange chromatography

Immunogen: Pyruvate Kinase [Rabbit Muscle].

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: ELISA (1:4000 - 1:20000), WB (1:500 - 1:2000)

Usage: Anti-Pyruvate Kinase Antibody has been assayed against 1.0 μg of Pyruvate Kinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:3000 to 1:15000 of the reconstitution concentration is suggested for Anti-Pyruvate Kinase Antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Rabbit

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 1 % BSA

Handling Advice: avoid freeze thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

Target Details

Target: Pyruvate Kinase, Muscle (PKM)

Alternative Name: PKM / Pyruvate Kinase, Muscle

Background: Name/Gene ID: PKM

Synonyms: PKM, p58, PK3, PKM2, Pyruvate Kinase, Pyruvate kinase 2/3, Pyruvate kinase, m1, Pyruvate kinase, muscle, TCB, Tumor M2-PK, OIP-3, OIP3, Opa-interacting protein 3, CTHBP, PK, muscle type, PK2, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, THBP1

Gene ID: 5315

Pathways: Warburg Effect