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NF-kB p65 antibody (Nuclear Factor-kB p65) (Thr435) Primary Antibody

NFkBP65 Reactivity: Human, Mouse IHC (p), WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN197113
$350.90
Plus shipping costs $45.00
50 μL ABIN197113
50 μL ABIN197113
local_shipping Shipping to: United States
Available
  • Target
    NF-kB p65 (NFkBP65)
    Binding Specificity
    • 36
    • 29
    • 27
    • 27
    • 27
    • 27
    • 23
    • 21
    • 21
    • 19
    • 16
    • 15
    • 15
    • 14
    • 13
    • 11
    • 11
    • 10
    • 8
    • 8
    • 6
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Thr435
    Reactivity
    • 547
    • 384
    • 310
    • 49
    • 44
    • 35
    • 17
    • 17
    • 13
    • 11
    • 7
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    Human, Mouse
    Host
    • 501
    • 47
    • 6
    • 1
    • 1
    Rabbit
    Clonality
    • 483
    • 71
    Polyclonal
    Conjugate
    • 299
    • 31
    • 22
    • 19
    • 18
    • 17
    • 17
    • 17
    • 17
    • 17
    • 13
    • 10
    • 10
    • 10
    • 10
    • 10
    • 7
    • 7
    • 2
    • 2
    • 1
    This NF-kB p65 antibody is un-conjugated
    Application
    • 354
    • 215
    • 157
    • 116
    • 107
    • 96
    • 92
    • 75
    • 68
    • 29
    • 23
    • 11
    • 10
    • 10
    • 10
    • 8
    • 7
    • 4
    • 2
    • 1
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Specificity
    NF-κB p65 antibody detects endogenous levels of total NF-κB p65 protein.
    Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
    Immunogen
    The antiserum was produced against synthesized non-phosphopeptide derived from human NF-kappaB p65 around the phosphorylation site of threonine 435 (E-G-TP-L-S).
  • Application Notes
    Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS(without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store the antibody undiluted at -20 °C.
  • Target
    NF-kB p65 (NFkBP65)
    Alternative Name
    RELA / NF-kB p65 (NFkBP65 Antibody Abstract)
    Synonyms
    C-Rel, NFKB3, p65, NFkB, c-rel, zgc:100833, Xrel2, Xrel3, rel, rel-A, rel2, rel3, v-rel, xrel, REL proto-oncogene, NF-kB subunit, RELA proto-oncogene, NF-kB subunit, v-rel reticuloendotheliosis viral oncogene homolog A (avian), v-rel avian reticuloendotheliosis viral oncogene homolog, v-rel avian reticuloendotheliosis viral oncogene homolog L homeolog, REL, RELA, Rela, Rel, rel, rel.L
    Background
    NFkB (Nuclear Factor NF-kappa-B) is a pleiotropic transcription factor that plays a role in many biological processes, including inflammation, immunity, differentiation, cell growth, tumorigenesis, and apoptosis. It is found as a homo- or heterodimeric complex containing the Rel-like domain containing proteins NFkB p65 (RELA/p65), RELB, NFkB1/p105, NFkB1/p50, REL and NFkB2/p52. The heterodimeric NFkB p65/p50 complex is the most abundant one. The dimers bind to kappa-B sites at their target genes, with the affinity of the interaction dependent on the subunit composition of the dimer. Furthermore, different dimers act as transcriptional activators or repressors, with the NFkB p65/p50 and p65-c-Rel complexes acting as activators. NFkB activity is controlled by several different mechanisms, including post-translational modifications, subcellular localisation and interactions with other coactivators or corepressors. NFkB complexes are held in the cytoplasm in an inactive state by interaction with members of the NFkB inhibitor (IkB) family. Typically, phosphorylation of IkB by IkB kinases (IKKs) in response to different activators leads to degradation of the inhibitor, allowing NFkB to translocate into the nucleus. The inhibitory effect of IkBs is primarily exerted through their interaction with NfKB p65. NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3. Invasion of a host by a pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of immune function required for resistance to infection.Synonyms: NF kappa B p65, NFKB3, NFkB p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, Rel A, Transcription factor p65
    Gene ID
    5970
    NCBI Accession
    NP_001138610
    UniProt
    Q04206
    Pathways
    NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins
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