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Ezrin antibody (Tyr353)

The Rabbit Polyclonal anti-Ezrin antibody has been validated for WB. It is suitable to detect Ezrin in samples from Human and Mouse.
Catalog No. ABIN197258
$558.00
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Quick Overview for Ezrin antibody (Tyr353) (ABIN197258)

Target

See all Ezrin (EZR) Antibodies
Ezrin (EZR)

Reactivity

  • 164
  • 83
  • 76
  • 8
  • 8
  • 7
  • 5
  • 4
  • 3
  • 3
  • 3
  • 1
  • 1
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Human, Mouse

Host

  • 147
  • 19
  • 2
  • 1
Rabbit

Clonality

  • 128
  • 41
Polyclonal

Conjugate

  • 98
  • 8
  • 4
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  • 4
  • 4
  • 4
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  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Ezrin antibody is un-conjugated

Application

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  • 59
  • 51
  • 19
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  • 13
  • 10
  • 10
  • 9
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB)
  • Binding Specificity

    • 28
    • 16
    • 10
    • 9
    • 9
    • 9
    • 6
    • 6
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    • 5
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    • 1
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    • 1
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    • 1
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    Tyr353

    Specificity

    Ezrin antibody detects endogenous levels of total Ezrin protein.

    Purification

    Affinity-chromatography using epitope-specific immunogen

    Immunogen

    The antiserum was produced against synthesized non-phosphopeptide derived from human Ezrin around the phosphorylation site of tyrosine 353 (Q-D-YP-E-E).
  • Application Notes

    Western Blot: 1: 500approx. 1000.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    1.0 mg/mL

    Buffer

    PBS(without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    -20 °C
  • Target

    Ezrin (EZR)

    Alternative Name

    Ezrin

    Background

    Ezrin (also known as p81, 80K, or cytovillin) is a cytoplasmic protein which is enriched in microvilli and other cell surface structures. Its NH2-terminal domain is associated with the plasma membrane and the COOH-terminal domain with the cytoskeleton. Ezrin has an actin-binding capability. Ezrin is expressed in epithelial cells but not in mesenchymal cells. Ezrin is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas. Ezrin serves as a tyrosine kinase substrate, and is phosphorylated in EGF-stimulated cells.Synonyms: Cytovillin, EZR, VIL2, Villin-2, p81

    Gene ID

    7430

    NCBI Accession

    NP_001104547

    UniProt

    P15311

    Pathways

    Maintenance of Protein Location
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